December 1, 2017 Presentation of a novel approach for antibody purification at the PEGS Europe 2017
A novel approach for antibody purification was recently presented by Anja Trapp, M.Sc., Scientist at Rentschler Biopharma, at this year's PEGS Europe Summit in Lisbon, Portugal.
The focus of the presentation was the use of caprylic acid as a precipitant. Caprylic acid is a well known substance in the pharma industry and has been used for decades to isolate immunglobulins from plasma. The hydrophobic molecules can interact with proteins inducing a precipitation. This mechanism can help to remove typical process- and product related impurities. Especially the low pH virus inactivation step enables a simple integration of capryl acid-induced precipitation. Using this approach, an additional host cell protein clearance of up to two logarithmic units is possible.
In a case study, it was shown that caprylic acid-induced precipitation can be implemented as an excellent alternative to column chromatography. It was possible to completely remove host cell proteins by the precipitation step. A third chromatographic column was no longer necessary to fulfill the final specifications.
In conclusion, the caprylic acid precipitation presents itself as a beneficial tool for the design of antibody purification processes. The benefits include minial costs and the opportunity utilizing existing tanks and process flows.
Rentschler Biopharma SE